To broaden the compass of pollutants covered in mortal- impacted riverine systems; water, deposition, and treated wastewater effluent were anatomized using receptor- grounded cell assays that give an intertwined response to chemicals grounded on their mode of natural exertion. Samples were collected from three California (USA) climaxes with varying degrees of urbanization and discharge from external wastewater treatment shops (WWTPs). To round cell assay results, samples were also anatomized for a suite of pollutants of arising concern (CECs) using gas and liquid chromatography- mass spectrometry (GC- and LC- MS/ MS).